Regulatory
Part:BBa_K1075044:Design
Designed by: Max Schelski Group: iGEM13_Bonn (2013-09-23)
Promoter(const.)-RBS34-YF1-FixJ-FixK-LambdaC-pC (pDawn)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
Design Notes
pDawn was designed by Moglich et al. [1]
Source
PCR amplification from pDawn, triple mutagenesis to remove EcoRI, PstI and XbaI
References
[http://www.sciencedirect.com/science/article/pii/S0022283612000113] Ohlendorf et al. (2009) From Dusk till Dawn One-Plasmid Systems for Light-Regulated Gene Expression.